The results of antimicrobial activity showed that water and ethanol crude extracts were active on all the strains of pathogens tested at different concentrations, with ethanol extracts exerting more activity. Antifungal activity of plants extracts were determined by agar plate methods. Review cytotoxic studies of plant extracts cytotoxicity of plant extracts on both plant and animal test material is capturing scientific interest, as it can indicate their antitumor and anticarcinogenic potentials, which have always been a gateway for the development of novel anticancer drugs. The phenol and flavonoid contents of the plant extracts were in the range 20. Antifungal activity of ginger extract on candida albicans. The components showed slight antimicrobial activity ahmed et al. Khamwan microbiology section of the central laboratory service unit faculty of medicine. Different dose of plant extracts 50, 100, 200 and 400 mgml were applied against these plant pathogens and the value of mycelial growth inhibition mgi and mycelial growth mg were recorded.
A comparative evaluation of antifungal activity of. About 50g of coarsely powdered plant materials 50g250ml were extracted in a soxhlet extractor for 8 to 10 hours, sequentially with petroleum ether, chloroform, ethyl acetate, methanol and water. Antioxidant activity of methanol extracts of different. Antibacterial activities of plant extracts were compared with those of standard antibiotics. Antibacterial activity of medicinal plant extracts. The analysis of bioactive compounds present in the plant extracts involving.
Pdf antibacterial and antifungal activity of plant extracts. Plant extracts with biofilm inhibitory activity biofilms are the default modeoflife for many bacterial species and biofilmbased infections. Extracts of capparis spinosa and juglans regia completely prevented growth of m. Determination of the mics of hydroalcoholic extracts of the 14 canadian botanicals against ng isolates revealed a range of inhibitory activities. Euphorbia fischeriana steud is a perennial herbaceous plant with latex, distributed mainly in north china 15. The present study aimed at evaluating the in vitro antimicrobial activity of methanolic extracts of some medicinal plants against escherichia coli, salmonella typhimurium, staphylococcus aureus and enterococcus sp. Antibacterial and antifungal activity of plant extracts 221 synthesis of the obtained results t able 6 showed that the activi ty of the extracts depended. In the present study, we evaluated the potential of 5 selected plants to inhibit test bacteria by agar well diffusion assay and the result is shown in table 2. Cold and hot water leaf extracts of 25 plant species comprising horticultural crops including multipurpose trees, weeds, forage crops and medicinal plants at 5% group i and 30 forest tree species at 10% group ii were tested against the brinjal aubergine, solanum melongena damping off pathogen, pythium aphanidermatum in pda cultures. All plant extracts used had shown antifungal activity against fusarium solani. Therefore, plant oils are widely used as a substrate for testing antioxidant activity. Antioxidant activity dpph free radical scavenging activity of methanolic extract the antioxidant activity of the plant extracts and the standard was assessed on the basis of the radical scavenging effect of the stable 1, 1diphenyl2picrylhydrazyl dpphfree radical activity by modified method.
All the extracts were assayed for their antibacterial activities in a standard minimum inhibitory concentration mic assay. Antioxidant activity in plant oil plant oils contain unsaturated fatty acids, which are most sensitive to oxidative degradation. Extracts of canadian first nations medicinal plants, used. Kijjoab, c abstract the ethyl acetate extracts of the culture of eupenicillium parvum, gelasinospora. Antifungal activity of wild sage lantana camara against colletotrichum falcatum a. These results show that the aqueous extracts of five native plants of. Evaluation of some plant extracts for their nematicidal properties against rootknot nematode, meloidogyne incognita m. The leaves were washed thor oughly 2 to 3 times with running tap water, leaf material. Antibacterial, antioxidant, and anticholinesterase activities of plant. Baskaran abstract nematicidal activities of extracts from plants were assayed against meloidogyne incognita.
Bioactive aqueous extracts of twelve yucatecan plants. An increased inhibitory effect of the mycelial growth was observed with the increase in concentration from 10 to 50%. Each purified extracts were dissolved in dimethyl sulfoxide, sterilized by filtration using sintered glass filter, and stored at 4c. Phytochemicals, antioxidant and antimicrobial potentials. Indeed, for use in food and nutraceuticals, aqueous plant extracts are nutritionally more relevant and would have obvious advantages in relation to certi. Punjaisee, department of clinical microbiology, faculty of associated medical sciences, chiang mai university c. The investigation of antimicrobial activity as well as cell toxicity of extracts from 30 plant species against five bacteria species and two fungi species was studied 29.
Consequently, the search and study of plant extracts that have the highest antiradical and antioxidant activity aoa is a very urgent task. Verma 1, pradeep babu 2, vinay gupta 2, gbks prasad 3 and v. Association of antibiotics and plant extracts showed synergistic antibacterial activity against antibioticresistant bacteria. Assessment of antioxidant activity of plant extracts. Antifungal activity of some medicinal plant extracts. Antibacterial activities of some borneo plant extracts. By ngaruiya paul njuguna a thesis submitted in partial fulfilment of master of. Preparation of plant extracts fresh plant material was washed under running tap water, air dried and powdered. Plant extracts have shown inhibitory effect on the growth of. Ten dif ferent plants were collected from in and around sivakasi area. In the present study, methanol and aqueous extracts of parkinsonia aculeata l.
The development of antibiotic resistant bacteria stems from a number of factors, including inappropriate use of antibiotics in human and animal. Antifungal activity of some plant extracts against. Evaluation of antioxidant activity of some plant extracts. Enzyme inhibitory properties, antioxidant activities, and. It was concluded that ethanol extracts from 70 % of the plants were toxic to cell and only one of the species of combretum duarteanum showed antimicrobial activity.
Evaluation of nematicidal activity of ethanol extracts of. Ethanolic crude extracts prepared from the arils and seeds, pericarp, peels and from the whole fruit of punica granatum, known as pomegranate, had their antifungal activity tested against candida spp. Pdf antifungal activity of some plant extracts on some. Phytochemical investigation and biological activity of. The capacity of the extracts and antibiotics were evaluated on the. Effect of combined extracts from different plant parts of. Antimicrobial activity of some plant extracts against. Antibacterial characheristics of plants from heracleum genus have relatively slightly been researched. Antimicrobial activity of wild olive crude extracts in vitro. None of the aqueous extracts showed antimicrobial activity. All plant extracts were screened for their fungistatic, fungicidal activities and minimum inhibitory dilution mid against above fungi. Antioxidant, antihypertensive, antihyperglycemic, and. Antifungal activity of some plant extracts on some pathogenic fungi article pdf available in archives of phytopathology and plant protection 473 february 2014 with 1,206 reads.
Trolox tr was used as a standard, and the totalantioxidant capacity of plant extracts were measured as mm tr equivalent. Antioxidant activity of plant extracts containing phenolic compounds. Antifungal activity of some medicinal plant extracts against candida albicans and cryptococcus neoformans s. Pdf antifungal activity of plant extracts with potential.
These data indicate that selected plant extracts are potential sources of dietary antioxidants. Efficacy of plant extracts in plant disease management. Evaluation of some plant extracts for their nematicidal. Several assays have been frequently used to estimate antioxidant capacities in. Briefly, a twodimensional, twoagent broth microdilution checkerboard technique was used to study the interaction between the promising extracts. Antioxidant properties of the methanol extracts of the.
Many plant extracts and essential oils have been screened for their nematicidal activity against the plantparasitic nematodes814. Antifungal activity of wild sage lantana camara against. The methanolic extract of caryophyllus aromaticus presented the highest antis. The antibacterial activity of plant extracts of the lamiaceae family was evaluated against clinical isolates of multiresistant gramnegative bacteria by broth. An invitro study abstract plant derived products have been used for medicinal purposes for centuries. This is a good study in which the authors evaluated the antioxidant activity of extracts of root, stem, leaf, flower and fruit of l. Numerous works devoted to antioxidant activity of plant extracts are published. For measuring radical scavenging activity rsa against the stable radical n,n diphenylnpicrylhydrazyl dpph, 0. The results showed that 16 extracts have antibacterial activity with more than 10 mm against a.
The extract from carrot was more heatstable than other extracts. Phytochemical analysis of phyllanthus amarus extracts s. Plant extracts 1 ml were allowed to react with 1 ml of the abts solution and the absorbance was taken at 734 nm after 7 min using the spectrophotometer. Antibacterial and antifungal activity of plant extracts 229 glaucus, aspergillus niger, botritis cinerea, cladosporium herbarum, fusarium culmorum, fusarium oxysporum, fusarium poae, fusarium. Results of antimicrobial activity of the five plant extracts can suggested that s. The abts scavenging capacity of the extract was compared with that of bht and percentage inhibition calculated as abts radical scavenging activity % abs control.
The antibacterial activity of extracts of nine plant species with good. Wild olive crude extracts were screened for antibacterial activity against five different bacterial human pathogens. The antifungal activity of extracts from datura stramonium, lawsonia alba, mentha piperita, eucalyptus citriodora, kigelia pinnata, lantana camara, azadirachta indica, canna indica, allium cepa, portulaca indica, ziziphus jujuba, prosopis spicigera, calotropis procera and other plants against alternaria brassicae, colletotrichum papayae and helminthosporium sp. Comparative study of antioxidant properties and total.
Antifungal activity of plant extracts with potential to. The effect of various plant extracts was measured on several important oxidation indicators. In vitro antifungal activity screening of crude extracts. The extracts that presented antimicrobial activity were tested for hemolytic assay against human a, b, and o blood types and rabbit blood. Assessment of antioxidant activity of plant extracts by. Screening of antibacterial activity of the medicinal plant. Antimicrobial activity of single and combined extracts of. In these works aoa are given in units % mg, mgml or mgg, that character. Aqueous, saline buffer and acid extracts of different plant species were screened in vitro for their antifungal activity against alternaria spp. Antifungal activity of some plant extracts against guava. Antioxidant activity was studied using dpph, cuprac, reducing power assay, deoxyribose degradation site and nonsite specific, ferric reducing antioxidant potential. Antifungal activity of plant extracts with potential to control plant pathogens in pineapple. Evidencebased complementary and alternative medicine. Methanol, ethylacetate extracts of plant species heracleum crenatifolium, h.
Studies on some plant extracts for their antimicrobial. Antifungal activity of plant extracts with potential to control plant pathogens in pineapple article pdf available in asian pacific journal of tropical biomedicine 61 november 2015 with. Antibacterial and antifungal activities from leaf extracts. Antifungal activity of some plant extracts against clinical pathogens abhishek mathur 1, ravinder singh 1, sajad yousuf 1, aprajita bhardwaj 1, satish k. Antimicrobial activity, toxicity and phytochemical. Antimycotic activity of the extract against the three dermatophytes varied significantly p test plants. Antibacterial and antifungal activities of plant part extracts against four pathogenic bacteria two grampositive and negative and three pathogenic fungi were investigated by the agar disk diffusion method.
Antibacterial activity of crude extracts of selected plants. A number of studies have investigated the activity of plant extracts and products against specific oral pathogens, while others have focused on the ability of the products to inhibit the formation of dental biofilms by reducing the adhesion of microbial pathogens to the tooth surface, which is a primary event in the formation of dental plaque. Extraction, isolation and characterization of bioactive compounds. Antifungal activity of plant extracts against dermatophytes article pdf available in mycoses 421112. In order to extract different phenolic compounds from plants with a high degree. The chemical examination follows after the isolation and biological screening of the active fraction1. Antibacterial activity of plant extracts 247 brazilian journal of microbiology 2000 31. Fresh leaves of eleven different plant species free from. Antioxidant activity of herbaceous plant extracts protect. As well, the smallest concentration capable of inhibiting or preventing growth was determined among the species and extracts that demonstrated inhibitory properties. The antifungal activity of the methanol extracts of six medicinal plants used in native medicine in sri lanka is reported. In traditional indian medicine or ayurveda, zingiber officinale and many other herbs have been used as medicine. Screening of in vitro antioxidant activity of methanolic. Antifungal activity of medicinal plant extracts against phytopathogenic fungus alternaria spp.
Phytochemical screening of plant extracts especially those which have been used in traditional medicine is therefore essential so as to identify phytochemical constituents in the plants that are responsible for a given bioactivity. Comparisons with pertinent data from literature indicate that, according to the methodology adopted in studies on antimicrobial activity, the most diverse results can be obtained. Four different solvents were used for the extraction. Effect of combined active plant parts extracts on antimicrobial activity combinations of promising extracts were assessed as previously described by berenbaum17. All plant hydroalcoholic extracts contained the expected phytochemical markers for each species, confirming their botanical identity data not shown. Antibacterial activity of lamiaceae plant extracts in clinical. In vitro antifungal activity screening of crude extracts of soil fungi against plant pathogenic fungi n. The differences of solvent related activity could be speculatively explained by the insolubility of active compounds in water.
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